From Basics To Mastery An Online Hplc Course

From Basics to Mastery: An Online HPLC Course for Analytical Scientists

What you'll learn

Develop a strong foundation in HPLC principles

Gain comprehensive knowledge of HPLC instrumentation

Master method development and optimization

Understand and apply method validation protocols

Enhance troubleshooting and system maintenance skills

Interpret chromatographic data and report results

Apply best laboratory practices and safety standards

Requirements

Analytical chemistry

Description

This online HPLC (High-Performance Liquid Chromatography) course, titled From Basics to Mastery: An Online HPLC Course for Analytical Scientists, is meticulously designed to provide a comprehensive, hands-on educational experience for participants seeking to develop both theoretical knowledge and practical skills in modern analytical laboratories. The course is structured to guide learners from foundational concepts to advanced mastery, making it relevant for students and professionals in pharmaceutical chemistry, analytical chemistry, toxicology, quality control, and related fields who aim to achieve proficiency in instrument operation, method development, and data interpretation. Participants will start by learning the basic ideas of chromatography, such as the simple concepts of thin-layer chromatography and the main ways that column chromatography separates substances. The course shows the benefits of HPLC compared to traditional column chromatography, helping learners understand the HPLC analysis process and the important tools used in this method. A close look at the key parts of an HPLC system—like the mobile-phase reservoir, pump, sample injection system, column, and detector—will be provided, along with a summary of common stationary phases and HPLC techniques. As the course progresses, learners engage with method development and optimization, utilizing schematic thinking, information resources, and practical procedures to design robust analytical methods. The course also looks at how to improve methods by considering factors like the column's material, particle size, length, and temperature, as well as the mobile phase's solvent mix, flow rate, and other techniques for quick method improvement. The course also teaches how to check if methods are working well, focusing on important performance factors and the steps needed to validate an analytical method, which includes collecting data, understanding chromatograms, and setting up software parameters. The course integrates practical sessions throughout, emphasizing HPLC system assembly, standard mix preparation, and hands-on HPLC setup and operation. Finally, the course provides essential training in troubleshooting and system maintenance, equipping participants with the skills to identify, diagnose, and resolve both common and serious HPLC problems, as well as maintain columns for optimal performance. By the end of the course, participants will be well-prepared to apply their knowledge in real-world laboratory settings, ensuring accurate and reliable analytical results.

Overview

Section 1: Introduction

Lecture 1 Introduction to HPLC and Chromatography Fundamentals

Section 2: Components of an HPLC System

Lecture 2 Components of an HPLC System

Section 3: Method Development and Optimization

Lecture 3 Method Development and Optimization

Section 4: Method Optimization Parameters

Lecture 4 Method Optimization Parameters

Section 5: Method validation

Lecture 5 Method validation

Section 6: Problem Shutdown and System Maintenance

Lecture 6 Problem Shutdown and System Maintenance

Section 7: PRACTICAL 1-HPLC FILTERATION UNIT

Lecture 7 PRACTICAL 1-HPLC FILTERATION UNIT

Section 8: HPLC Configuration

Lecture 8 HPLC configuration

Lecture 9 Run HPLC system

Graduate student chemistry pharmacy toxicology,Professionals,Researchers,Undergraduate students