Celiac Disease: Methods and Protocols
Michael N. Marsh - Celiac Disease: Methods and Protocols
Published: 2000-04-01 | ISBN: 0896036502, 1617371157 | PDF | 288 pages | 3 MB
Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.
Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.