Cytochrome P450 Protocols (Methods in Molecular Biology) by Ian R. Phillips
English | July 30, 1998 | ISBN: 0896035190 | 469 Pages | PDF | 30 MB
English | July 30, 1998 | ISBN: 0896035190 | 469 Pages | PDF | 30 MB
Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques and invaluable classic methods for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphism.