Electro Microscopy Methods and Protocols (repost)
Electro Microscopy Methods and Protocols (Methods in Molecular Biology) by M. A. Nasser Hajibagheri
English | ISBN: 0896036405 | edition 1999 | PDF | 277 pages | 3,7 mb
English | ISBN: 0896036405 | edition 1999 | PDF | 277 pages | 3,7 mb
Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations.